Typhi resulted in reduced intracellular bacterial recovery and highly enhanced protection against S. Treatment of mice with Au-VG16KRKP post-infection with S. Typhi LPS under both in vitro and in vivo conditions. Au-VG16KRKP can penetrate host epithelial and macrophage cells as well as interact with intracellular S. In comparison with the free VG16KRKP peptide or gold nanoparticle alone, the conjugated variant, Au-VG16KRKP, is non-cytotoxic to eukaryotic cells, but exhibits strong bacteriolytic activity in culture. Here, we have studied the mechanism of action of a designed antimicrobial peptide, VG16KRKP (VARGWKRKCPLFGKGG), delivered via gold nanoparticle tagging against Salmonella infection by combining biological experiments with high- and low-resolution spectroscopic techniques. Although nanoparticle-tagged antimicrobal peptides have gained considerable importance in recent years, their structure-function correlation has not yet been explored.
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